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Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

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Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
09:54

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Published on: April 18, 2019

Stem cells and tissue-engineered skin.

A Charruyer1, R Ghadially

  • 1Department of Dermatology, University of California and Veterans Affairs Medical Center, San Francisco, CA, USA.

Skin Pharmacology and Physiology
|February 4, 2009
PubMed
Summary
This summary is machine-generated.

Tissue-engineered skin using epidermal stem cells offers improved wound healing and gene therapy for skin diseases. Optimizing stem cell isolation and culture enhances engineered skin quality and longevity.

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Area of Science:

  • Regenerative Medicine
  • Dermatology
  • Biotechnology

Background:

  • Clinical applications in wound healing and gene therapy require advancements in skin tissue engineering.
  • Epidermal stem cells are crucial for developing improved tissue-engineered skin constructs.

Purpose of the Study:

  • To review the use of epidermal stem cells for enhanced skin tissue engineering.
  • To discuss methods for isolating pure epidermal stem cell preparations.
  • To explore culture conditions for maintaining stem cell purity and optimizing engineered skin.

Main Methods:

  • Review of current literature on epidermal stem cell isolation and culture techniques.
  • Analysis of biomarker-based isolation strategies and their limitations.
  • Evaluation of culture conditions for maintaining stem cell purity and function.

Main Results:

  • Biomarker-based isolation of epidermal stem cells is important but has limitations in achieving purity.
  • Specific culture conditions are necessary to maintain stem cell purity for superior engineered skin.
  • Utilizing additional multipotent stem cell sources can optimize engineered skin functionally and cosmetically.

Conclusions:

  • Epidermal stem cells are a promising source for advanced skin tissue engineering.
  • Optimized isolation and culture are key to developing high-quality, long-lasting engineered skin.
  • Combining epidermal stem cells with other multipotent stem cells offers further functional and cosmetic benefits.