Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme Kinetics

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Foam Formation in Shake Flasks and Its Consequences.

Engineering in life sciences·2025

Same author

Online monitoring of the mitochondrial respiration activity and protein formation in the Almost Living Cell-free Expression (ALiCE) system.

BMC biotechnology·2025

Same author

Application of online spectroscopic techniques for detection of Bacillus spores in cultivations with complex medium.

Journal of microbiological methods·2025

Same author

Simultaneous online monitoring of viscosity and oxygen transfer rate in shake flask cultures.

Journal of biological engineering·2025

Same author

Primary high-throughput screening of engineered phytases by online monitoring of the oxygen transfer rate of Komagataella phaffii.

Microbial cell factories·2025

Same author

Monoclonal antibody neutralizes <i>Staphylococcus aureus</i> serine protease-like protein B (SplB)-induced pathology.

Infection and immunity·2025

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

"Enzyme Test Bench," a high-throughput enzyme characterization technique including the long-term stability.

Kirill Rachinskiy¹, Hergen Schultze, Matthias Boy

¹Department of Biochemical Engineering, Sammelbau Biologie, RWTH-Aachen University, D-52074 Aachen, Germany.

Biotechnology and Bioengineering

|February 4, 2009

Summary

A new Enzyme Test Bench technique rapidly predicts enzyme stability using accelerated testing under extreme conditions. This method accurately forecasts long-term enzyme performance, optimizing enzyme selection and process temperature for industrial applications.

More Related Videos

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Published on: March 26, 2019

Measuring In Vitro ATPase Activity for Enzymatic Characterization

Measuring In Vitro ATPase Activity for Enzymatic Characterization

Published on: August 23, 2016

Related Experiment Videos

Last Updated: Jun 25, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Published on: March 26, 2019

Measuring In Vitro ATPase Activity for Enzymatic Characterization

Measuring In Vitro ATPase Activity for Enzymatic Characterization

Published on: August 23, 2016

Area of Science:

Biotechnology
Enzyme Engineering
Biochemical Engineering

Background:

Enzyme characterization is crucial for industrial applications.
Predicting long-term enzyme stability under operational conditions is challenging.
Current methods for stability assessment are often time-consuming.

Purpose of the Study:

To present a novel high-throughput technique, the Enzyme Test Bench, for rapid enzyme characterization.
To predict long-term enzyme stability under moderate conditions using short-term tests under extreme conditions.
To enable optimization of enzyme selection and process temperature.

Main Methods:

Utilized mathematical modeling of temperature-dependent enzyme kinetics (activation/deactivation).
Employed optimal non-linear experimental design to accelerate deactivation effects.
Developed automated instrumentation for simultaneous fluorescent assays, mixing, and precise temperature control (10-85°C).
Established a universal fluorescent assay for ester hydrolysis via pH-shift.

Main Results:

Short-term experiments (hours) in microtiter plates accurately predicted long-term stability (weeks) in larger reactors.
The technique successfully characterized two esterases, correlating short-term and long-term data.
Demonstrated that simple activity measurements after heat exposure can lead to suboptimal enzyme choices.

Conclusions:

The Enzyme Test Bench offers a rapid and reliable method for enzyme screening and stability prediction.
Accurate temperature-dependent characterization is essential for informed enzyme selection and process optimization.
This approach enhances efficiency in enzyme discovery and application development.