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Updated: Jun 25, 2026
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
Published on: June 17, 2016
Obstructive apneas induce early release of mesenchymal stem cells into circulating blood
Alba Carreras1, Isaac Almendros, Irene Acerbi
1Unitat de Biofisica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona--IDIBAPS, Barcelona, Spain.
Study Objectives:
To investigate whether noninvasive application of recurrent airway obstructions induces early release of mesenchymal stem cells into the circulating blood in a rat model of obstructive sleep apnea.
Design:
Prospective controlled animal study.
Setting:
University laboratory.
Patients Or Participants:
Twenty male Sprague-Dawley rats (250-300 g).
Interventions:
A specially designed nasal mask was applied to the anesthetized rats. Ten rats were subjected to a pattern of recurrent obstructive apneas (60 per hour, lasting 15 seconds each) for 5 hours. Ten anesthetized rats were used as controls.
Measurements And Results:
Mesenchymal stem cells from the blood and bone marrow samples were isolated and cultured to count the total number of colony-forming unit fibroblasts (CFU-F) of adherent cells after 9 days in culture. The number of CFU-F from circulating blood was significantly (P = 0.02) higher in the rats subjected to recurrent obstructive apneas (5.00 +/- 1.16; mean +/- SEM) than in controls (1.70 +/- 0.72). No significant (P = 0.54) differences were observed in CFU-F from bone marrow.
Conclusions:
Application of a pattern of airway obstructions similar to those experienced by patients with sleep apnea induced an early mobilization of mesenchymal stem cells into circulating blood.
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