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Related Experiment Videos

IL-4-dependent IgE switch in membrane IgA-positive human B cells.

X H Zhang1, C Werner-Favre, H Y Tang

  • 1Department of Medicine, Geneva University Hospital, Switzerland.

Journal of Immunology (Baltimore, Md. : 1950)
|November 1, 1991
PubMed
Summary

Human B cells expressing IgM or IgA frequently switch to producing IgE, while IgG-expressing B cells rarely do. This IL-4 dependent process is significant for IgE production in mucosal tissues.

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Immunoglobulin E (IgE) plays a crucial role in allergic reactions and defense against parasites.
  • B cell differentiation and immunoglobulin class switching are complex processes influenced by various cytokines.

Purpose of the Study:

  • To investigate the capacity of human B cells expressing different membrane immunoglobulin (Ig) isotypes to produce IgE.
  • To determine the role of Interleukin-4 (IL-4) in IgE production by distinct B cell populations.

Main Methods:

  • Utilized a clonal assay with EL-4 thymoma cells, T cell supernatant, and recombinant IL-4 (rIL-4).
  • Employed the autoclone apparatus of Fluorescence-Activated Cell Sorting (FACS) to analyze IgE responses from B cells separated by membrane Ig classes (IgM, IgG, IgA).

Main Results:

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  • IgE responses were frequently generated by IgM-positive (16%) and IgA-positive (14%) B cells, but rarely by IgG-positive B cells (1.3%).
  • All IgE secretion was dependent on IL-4.
  • Evidence suggests the existence of in vivo IgE-committed B cells co-expressing membrane IgA and IgE (IgA+E).

Conclusions:

  • IL-4 dependent IgE switching occurs frequently in IgM- and IgA-positive B cells, but rarely in IgG-positive B cells.
  • The findings suggest that IgA-expressing B cells in mucosal tissues may be a significant source of IgE production.