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One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
Published on: June 18, 2016
The interaction between Helicobacter pylori and atopy: does inverse association really exist?
Sebahat Cam1, Deniz Ertem, Nerin Bahceciler
1Marmara University School of Medicine, Istanbul, Turkey.
Helicobacter
|February 5, 2009
Summary
Helicobacter pylori infection and atopy show a counteractive immune response involving T helper cells. However, this immune balance does not prevent atopic diseases in children.
Area of Science:
- Immunology
- Microbiology
- Pediatrics
Background:
- Cross-sectional and case-control studies suggest an inverse association between Helicobacter pylori infection and atopic diseases.
- The underlying immunologic mechanisms of this association remain understudied.
Purpose of the Study:
- To investigate T helper (Th) cell function in H. pylori-infected children.
- To compare cytokine responses in atopic and non-atopic children with and without H. pylori infection.
Main Methods:
- Recruited 74 children from a 6-year cohort, assessing H. pylori status and atopy via questionnaire and skin-prick testing.
- Isolated peripheral blood mononuclear cells from 34 children and cultured with H. pylori, Der p 1, and phytohemagglutinin (PHA).
- Measured levels of interferon-gamma (IFN-gamma), interleukin (IL)-4, IL-10, and transforming growth factor-beta (TGF-beta).
Main Results:
- H. pylori-infected children showed higher IFN-gamma production in response to PHA and H. pylori, but this was suppressed when atopy was also present.
- Atopic children had higher PHA and Der p 1-induced IL-4 levels, which were suppressed by H. pylori coinfection.
- TGF-beta production was suppressed in atopic children, regardless of H. pylori status.
Conclusions:
- H. pylori infection and atopy exhibit a counteractive interaction between Th1 and Th2 cytokine responses.
- This immunologic counterbalance, however, did not confer protection against the development of atopy.
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