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Updated: Jun 25, 2026
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Methyl-binding DNA capture Sequencing for Patient Tissues
Published on: October 31, 2016
Methylation profiles in breast cancer
Nur Buyru1, Julide Altinisik, Filiz Ozdemir
1Department of Medical Biology, Istanbul University Cerrahpasa Medical Faculty, Istanbul, Turkey.
Cancer Investigation
|February 6, 2009
Summary
Aberrant methylation of tumor-suppressor genes is common in breast cancer. Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) effectively analyzes these epigenetic changes for potential diagnostic and therapeutic applications.
Area of Science:
- Oncology
- Epigenetics
- Molecular Biology
Background:
- Aberrant promoter methylation of tumor-suppressor genes is a hallmark of cancer.
- Identifying these epigenetic alterations is crucial for understanding breast cancer development and progression.
Purpose of the Study:
- To assess aberrant methylation of multiple tumor-suppressor genes simultaneously in breast cancer.
- To evaluate the utility of methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) for analyzing these epigenetic alterations.
Main Methods:
- Analysis of breast tumor and normal tissue samples from 77 patients.
- Utilized methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) to detect promoter methylation in 24 selected genes.
Main Results:
- Promoter methylation was observed in 17 out of 24 genes across tumor samples.
- RASSF1 and GSTP1 were the most frequently methylated genes, followed by DAPK1 and CDKN2B.
- Specific gene methylation was a frequent event in the studied breast cancer cohort.
Conclusions:
- Aberrant methylation of tumor-suppressor genes is a common event in breast cancer.
- MS-MLPA is a powerful and efficient tool for analyzing widespread epigenetic alterations.
- These findings support the potential use of MS-MLPA in the diagnosis and therapeutic strategies for breast cancer.
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