Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microwave biosensor for amylase detection in drainage fluid to monitor anastomotic leakage.

Biosensors & bioelectronics·2025
Same author

A Versatile Droplet Microfluidic Platform Capable of Confining Preformed Spheroids in Hydrogel Microenvironments for Downstream Growth and Analysis.

ACS biomaterials science & engineering·2025
Same author

Soft Dynamic Fluidic Cushion for Pressure Sore Management in Transtibial Prosthetics: A Proof-of-Concept Study.

IEEE transactions on bio-medical engineering·2025
Same author

Size and concentration characterization of microplastic particles in aqueous samples using sensitivity-enhanced coupled planar microwave resonators.

Journal of hazardous materials·2025
Same author

A functionalized microwave biosensor for rapid, reagent-free detection of E. coli in water samples.

Biosensors & bioelectronics·2025
Same author

Design Guidelines of Free-Flow Counterflow Gradient Focusing Device for Protein Fractionation.

Electrophoresis·2025

Related Experiment Video

Updated: Jun 25, 2026

Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique
10:12

Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique

Published on: June 12, 2015

Numerical and experimental evaluation of microfluidic sorting devices.

Jay K Taylor1, Carolyn L Ren, G D Stubley

  • 1Dept. of Mechanical and Mechatronics Engineering, University of Waterloo, Waterloo, ON, Canada N2L 3G1.

Biotechnology Progress
|February 6, 2009
PubMed
Summary

This study enhances lab-on-a-chip cell sorting devices with design modifications for portable, reliable operation. New features improve flow control and reduce energy needs for efficient bioparticle separation.

More Related Videos

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Related Experiment Videos

Last Updated: Jun 25, 2026

Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique
10:12

Analyzing Mixing Inhomogeneity in a Microfluidic Device by Microscale Schlieren Technique

Published on: June 12, 2015

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Area of Science:

  • Biotechnology
  • Microfluidics
  • Analytical Chemistry

Background:

  • Lab-on-a-chip devices require efficient bioparticle and cell isolation.
  • Existing cell-sorting designs face limitations in applied voltage, efficiency, reliability, and size.
  • Further microfluidic chip design improvements are crucial for integrating into larger systems.

Purpose of the Study:

  • To evaluate design modifications for reducing applied potential in portable cell-sorting devices.
  • To enhance operational reliability by minimizing pressure disturbances during electrokinetic pumping.
  • To improve flow control for dynamic sorting and counting of bioparticles.

Main Methods:

  • Fabrication of microfluidic chips in glass and polymeric materials.
  • Incorporation of asymmetric channel widths for sample focusing.
  • Utilizing nonuniform channel depth, directing streams, and online filters for optimized performance.
  • Employing fluorescence-based visualization for experimental validation.
  • Conducting numerical simulations using COMSOL, validated against experimental data.

Main Results:

  • Demonstrated reduction in required applied potential for portable device development.
  • Achieved improved operational reliability by minimizing induced pressure disturbances.
  • Showcased enhanced flow control with directing streams for dynamic sorting and counting.
  • Validated chip design advantages through fluorescence-based visualization and COMSOL simulations.

Conclusions:

  • Specific microfluidic chip design modifications significantly improve cell-sorting device performance.
  • The developed designs contribute to the creation of portable, reliable, and efficient lab-on-a-chip systems.
  • Integration of asymmetric channels, nonuniform depth, directing streams, and filters enhances bioparticle separation and control.