Automation of the acclimation phase in a sequencing batch reactor using dissolved oxygen regulation
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Updated: Jun 25, 2026

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
Published on: May 18, 2020
Alejandro Vargas1, Germán Buitrón
1Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Querétaro, Mexico. avargasc@il.unam.mx
Abstract:
In this work, an automatic strategy for acclimation of biomass to toxic wastewater biodegradation in a sequencing batch reactor (SBR) is proposed and evaluated. It is based on monitoring the airflow needs for regulating the dissolved oxygen concentration at a desired set point. The regulation is achieved by using a PID controller with an input/output linearizing feedforward part, using the airflow rate as input variable. A simple algorithm to detect the end of the reaction phase using this manipulated signal is proposed. The strategy was tested on a laboratory bioreactor using 4-chlorophenol as model toxic compound and two inocula from different sources. The results show that it is a viable alternative for automatic acclimation of biomass for toxic wastewater treatment in a SBR. Furthermore, the strategy is also suitable for the operation of the reactor after acclimation.
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