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Updated: Jun 25, 2026

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
Published on: May 18, 2020
Automation of the acclimation phase in a sequencing batch reactor using dissolved oxygen regulation
Alejandro Vargas1, Germán Buitrón
1Laboratory for Research on Advanced Processes for Water Treatment, Instituto de Ingeniería, Unidad Académica Juriquilla, Universidad Nacional Autónoma de México, Querétaro, Mexico. avargasc@il.unam.mx
Abstract:
In this work, an automatic strategy for acclimation of biomass to toxic wastewater biodegradation in a sequencing batch reactor (SBR) is proposed and evaluated. It is based on monitoring the airflow needs for regulating the dissolved oxygen concentration at a desired set point. The regulation is achieved by using a PID controller with an input/output linearizing feedforward part, using the airflow rate as input variable. A simple algorithm to detect the end of the reaction phase using this manipulated signal is proposed. The strategy was tested on a laboratory bioreactor using 4-chlorophenol as model toxic compound and two inocula from different sources. The results show that it is a viable alternative for automatic acclimation of biomass for toxic wastewater treatment in a SBR. Furthermore, the strategy is also suitable for the operation of the reactor after acclimation.
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