Related Experiment Videos
Determination of hydroxylysine in urine
The Tohoku Journal of Experimental Medicine
|February 1, 1977
Summary
A novel colorimetric method quantifies urinary hydroxylysine. This technique accurately measures hydroxylysine excretion, revealing an average adult excretion rate of approximately 110 micromoles per day.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Urology
Background:
- Hydroxylysine is a key component of collagen.
- Accurate measurement of hydroxylysine in urine is important for understanding collagen metabolism and related diseases.
- Existing methods for urinary hydroxylysine determination can be complex and time-consuming.
Purpose of the Study:
- To develop and validate a new, sensitive colorimetric method for the quantitative determination of hydroxylysine in human urine.
- To establish the normal urinary excretion rate of hydroxylysine in adults.
Main Methods:
- A 24-hour urine sample was processed, diluted, and acidified.
- Column chromatography (Dowex 50 X 4) and preparative paper chromatography were used for isolation and purification of hydroxylysine.
- The isolated hydroxylysine was oxidized with sodium metaperiodate, and the liberated formaldehyde was assayed colorimetrically using chromotropic acid reagents.
Main Results:
- The method successfully separated hydroxylysine from other amino acids, particularly serine and threonine.
- The colorimetric assay provided a reliable quantification of hydroxylysine.
- The average daily urinary excretion rate of hydroxylysine in adults was determined to be approximately 110 micromoles per day.
Conclusions:
- A new, efficient colorimetric method for urinary hydroxylysine determination has been established.
- This method offers improved separation and quantification of hydroxylysine.
- The findings provide a valuable reference for normal hydroxylysine excretion levels in adults.