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Updated: Jun 25, 2026

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
Published on: February 13, 2019
Electronic detection of DNA mutation based on strand exchange reaction
Mariko Watanabe1, Satoshi Kumamoto, Mitsunobu Nakamaura
1Department of Materials Science and Chemistry, University of Hyogo, Himeji, Hyogo, Japan.
Abstract:
A new approach to electronic detection of a single base mismatch is described. The assay involves the electrochemical measurements of DNA strand exchange reactions (SERs) between electrode-bound redox-modified DNA duplex and target DNA, where the sequence of redox-modified DNA is exchangeable to that of the target DNA. The presence of a single base mismatch can be determined from the slower SER rates compared with fully matched DNA.
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