Demonstration of lipids in plastic resin-embedded sections of skin material

Two different fluorescence stains, green: 5-hexadecanoylaminofluorescein, and red: BODIPY(R) 665/676 [(E,E)-3, 5-bis-(4-phenyl-1,3-butadienyl)-4,4-difluoro-4-bora-3a, 4a-diaza-s-indacene, produced good results regarding the demonstration of glycolipids, free fatty acids and triglycerides in mammalian skin material that had been embedded in a water miscible plastic resin (Technovit(R) 7100). In this way, functional aspects of specific structures (epidermal barrier region, sebaceous glands) could be characterized histochemically in the integument of five mammalian species with sparse or dense hair coats.