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Updated: Jun 25, 2026

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Published on: October 2, 2017

Aberration-corrected microscopy for structural biology applications.

A Ziegler¹, B Rockel, R Hegerl

¹Max-Planck Institute of Biochemistry, Molecular Structural Biology, D-82152 Martinsried, Germany. ziegler@biochem.mpg.de

Journal of Microscopy

|February 7, 2009

Summary

This summary is machine-generated.

This study investigated C(s)-corrected transmission electron microscopy for biological samples. Results show C(s) correction does not significantly impact typical imaging conditions for macromolecular complexes.

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Area of Science:

Structural biology
Electron microscopy
Macromolecular complex analysis

Background:

Transmission electron microscopy (TEM) is crucial for high-resolution structural studies of biological samples.
Image resolution in biological TEM is heavily influenced by defocus contrast.
C(s) correctors aim to improve resolution by correcting for spherical aberration.

Purpose of the Study:

To evaluate the utility of a C(s)-corrected 300-kV TEM for structural analysis of isolated macromolecular complexes.
To determine if large defocus imaging, common in biological studies, benefits from C(s) correction.

Main Methods:

Utilized a 300-kV transmission electron microscope equipped with a C(s) corrector.
Recorded image sets at various defocus and C(s) settings.
Compared experimental results with theoretical frameworks.

Main Results:

Experimental findings align well with theoretical predictions.
C(s) correction was found to have no significant effect on image resolution under typical biological imaging conditions.
Defocus contrast remains the dominant factor influencing resolution.

Conclusions:

C(s) correction is not essential for standard structural studies of biological samples using transmission electron microscopy under typical imaging conditions.
The benefits of C(s) correction may be limited for routine macromolecular complex analysis where defocus contrast is paramount.