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Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
Published on: December 6, 2018
Nocardioides sediminis sp. nov., isolated from a sediment sample
Syed G Dastager1, Jae-Chan Lee, Yoon-Jung Ju
1Korea Research Institutes of Biosciences and Biotechnology, 52 Eoeun-dong, Yuseong gu, Daejeon 305-333, Republic of Korea.
International Journal of Systematic and Evolutionary Microbiology
|February 7, 2009
Summary
A novel actinomycete bacterium, Nocardioides sediminis MSL-01(T), was discovered in Korean sediment. This strictly aerobic, Gram-positive bacterium represents a new species within the Nocardioides genus.
Area of Science:
- Microbiology
- Bacteriology
- Actinobacteria
Background:
- Actinomycetes, particularly the genus Nocardioides, are known for their diverse metabolic capabilities and presence in various environments.
- Sediment ecosystems harbor a vast, largely uncharacterized microbial diversity, including potential novel bacterial species.
Purpose of the Study:
- To isolate and characterize a novel actinomycete strain from a marine sediment sample.
- To determine the taxonomic position of the novel isolate using polyphasic characterization, including 16S rRNA gene sequencing and DNA-DNA hybridization.
- To propose a new species designation for the novel isolate based on distinct phylogenetic and phenotypic properties.
Main Methods:
- Isolation of strain MSL-01(T) from a sediment sample collected in Korea.
- 16S rRNA gene sequence analysis for phylogenetic placement.
- DNA-DNA relatedness studies to assess genomic similarity with closely related species.
- Analysis of chemotaxonomic markers, including cell-wall peptidoglycan composition, major menaquinone, and fatty acid profiles.
Main Results:
- A strictly aerobic, motile, short-rod-shaped, Gram-positive actinomycete strain, MSL-01(T), was isolated.
- Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MSL-01(T) belongs to the genus Nocardioides, with highest similarity to Nocardioides terrigena DS-17(T) (98.54%).
- Low DNA-DNA relatedness (34%) between strain MSL-01(T) and N. terrigena DS-17(T), along with distinct physiological properties and chemotaxonomic data (ll-diaminopimelic acid, MK-8(H(4)), iso-C(16:0)), supported its classification as a new species.
Conclusions:
- Strain MSL-01(T) represents a novel species within the genus Nocardioides, proposed as Nocardioides sediminis sp. nov.
- The distinct phylogenetic and phenotypic characteristics differentiate Nocardioides sediminis from its closest relatives.
- This finding expands the known diversity of the Nocardioides genus and highlights the potential for discovering novel microorganisms in marine sediment environments.
