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Updated: Jun 25, 2026

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
Published on: May 17, 2014
Elongation dynamics shape bursty transcription and translation
Maciej Dobrzynski1, Frank J Bruggeman
1Centrum Wiskunde & Informatica, Kruislaan 413, 1098 SJ, Amsterdam, The Netherlands.
Abstract:
Cells in isogenic populations may differ substantially in their molecular make up because of the stochastic nature of molecular processes. Stochastic bursts in process activity have a great potential for generating molecular noise. They are characterized by (short) periods of high process activity followed by (long) periods of process silence causing different cells to experience activity periods varying in size, duration, and timing. We present an analytically solvable model of bursts in molecular networks, originally developed for the analysis of telecommunication networks. We define general measures for model-independent characterization of bursts (burst size, significance, and duration) from stochastic time series. Inspired by the discovery of bursts in mRNA and protein production by others, we use those indices to investigate the role of stochastic motion of motor proteins along biopolymer chains in determining burst properties. Collisions between neighboring motor proteins can attenuate bursts introduced at the initiation site on the chain. Pausing of motor proteins can give rise to bursts. We investigate how these effects are modulated by the length of the biopolymer chain and the kinetic properties of motion. We discuss the consequences of those results for transcription and translation.
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