Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Distribution: Tissue Binding01:21

Drug Distribution: Tissue Binding

Upon entering the systemic circulation, drugs can distribute into the interstitial and intracellular fluid of various tissue cells. This distribution is facilitated by the binding of drugs to different cellular components within tissues, which may lead to drug accumulation in specific areas. Drugs bound to tissue components serve as reservoirs that release free drugs back into the system, prolonging the drug's overall action. However, this accumulation can also result in local toxicity.
For...
Antibiotic Selection00:57

Antibiotic Selection

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Obtaining source material for cellular agriculture.

Heliyon·2024
Same author

What Is the Standard Volume to Increase a Cup Size for Breast Augmentation Surgery? A Novel Three-Dimensional Computed Tomographic Approach.

Plastic and reconstructive surgery·2017
Same author

Analysis of the cephalometric changes in the first 3 months after spring-assisted cranioplasty for scaphocephaly.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2017
Same author

Evaluation of the artificial cotton roll tendon model for mechanical tendon repair experiments.

The Journal of hand surgery, European volume·2016
Same author

The histological and elemental characterisation of corrosion particles from taper junctions.

Bone & joint research·2016
Same author

Looped suture properties: implications for multistranded flexor tendon repair.

The Journal of hand surgery, European volume·2015

Related Experiment Video

Updated: Jun 25, 2026

Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection
09:09

Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection

Published on: March 14, 2019

Antibiotic distribution in a bone cement spacer model.

K Shiramizu1, V Lovric, A M D Leung

  • 1Surgical and Orthopaedic Research Laboratories, University of New South Wales, Prince of Wales Hospital, Randwick, NSW, Australia. keishiramizu@gmail.com

Hip International : the Journal of Clinical and Experimental Research on Hip Pathology and Therapy
|February 7, 2009
PubMed
Summary

Hanssen's technique for mixing antibiotics into bone cement, regardless of cement viscosity, ensures even antibiotic distribution. This method is suitable for high-dose antibiotic preparations, simplifying the process for better clinical outcomes.

More Related Videos

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

Related Experiment Videos

Last Updated: Jun 25, 2026

Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection
09:09

Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection

Published on: March 14, 2019

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
06:18

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials

Published on: March 3, 2023

Area of Science:

  • Orthopedic surgery
  • Biomaterials science
  • Pharmacology

Background:

  • High-dose antibiotics are mixed into bone cement for localized infection treatment.
  • Traditional mixing methods can be challenging with antibiotic powders.
  • Hanssen's technique involves mixing antibiotics during the cement's liquid phase.

Purpose of the Study:

  • To evaluate the impact of cement mixing technique on antibiotic distribution.
  • To assess the influence of cement viscosity on antibiotic distribution.
  • To compare traditional versus Hanssen's technique for antibiotic-cement mixtures.

Main Methods:

  • Three groups of cylindrical bone cement models were created.
  • Group A: Traditional technique, medium viscosity cement.
  • Group B: Hanssen's technique, medium viscosity cement.
  • Group C: Traditional technique, low viscosity cement.
  • Tetracycline distribution was analyzed using fluorescent microscopy and pixel analysis.
  • Distribution disequilibrium was quantified by comparing cross-section pixel counts.

Main Results:

  • No significant differences in antibiotic distribution were found between the traditional and Hanssen's techniques.
  • No significant differences were observed when comparing medium and low viscosity cements using the traditional technique.
  • Both mixing methods and cement viscosities yielded comparable antibiotic distribution.

Conclusions:

  • Hanssen's technique is a viable method for incorporating high-dose antibiotics into bone cement.
  • Both medium and low viscosity cements are suitable for antibiotic distribution when mixed appropriately.
  • The study supports the use of Hanssen's technique for improved ease of antibiotic-cement preparation without compromising distribution.