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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...

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On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Bioanalytical separations using electric field gradient techniques.

Michelle M Meighan1, Sarah J R Staton, Mark A Hayes

  • 1Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-1604, USA.

Electrophoresis
|February 7, 2009
PubMed
Summary
This summary is machine-generated.

New electric-field-gradient strategies are revolutionizing separations science for diverse targets like cells and molecules. This review covers emerging techniques and applications from 2007-2008, highlighting over 100 new contributions.

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Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

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On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

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Published on: March 2, 2012

Electrophoretic Separation of Proteins
08:17

Electrophoretic Separation of Proteins

Published on: June 12, 2008

Area of Science:

  • Separations Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Electric field gradient (EFG) techniques represent an emerging area in separations science.
  • These methods offer novel capabilities for analyzing a wide range of targets, from particles to living cells.

Purpose of the Study:

  • To review the diverse landscape of EFG-based separation techniques.
  • To categorize these techniques based on their analytical targets.
  • To provide an overview of recent advancements and applications.

Main Methods:

  • Literature review of contributions from early 2007 to mid-2008.
  • Categorization of techniques including dielectrophoresis, electric field gradient focusing (dynamic, true moving bed, pulsed field), electrocapture, electrophoretic focusing, temperature gradient focusing, and centrifugal force focusing.

Main Results:

  • The field has seen rapid development with approximately 100 new contributions in a short period.
  • Techniques are applicable to a broad spectrum of analytical targets, including small molecules, particles, soot, and living cells.
  • Emerging applications demonstrate the versatility of EFG-based separations.

Conclusions:

  • EFG techniques are poised to significantly impact the field of separations science.
  • The diversity of methods and applications underscores the dynamism and potential of this research area.
  • Continued innovation in EFG strategies promises further advancements in analytical capabilities.