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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jun 25, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantitative interaction proteomics using mass spectrometry.

Alexander Wepf1, Timo Glatter, Alexander Schmidt

  • 1Institute of Molecular Systems Biology, Swiss Federal Institute of Technology, Eidgenössische Technische Hochschule Zürich, Wolfgang Pauli-Strasse 16, Zurich, Switzerland.

Nature Methods
|February 10, 2009
PubMed
Summary
This summary is machine-generated.

We developed a mass spectrometry method for precise protein complex quantification. This approach accurately measures protein interactions within complex biological networks like human protein phosphatase 2A.

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A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
14:44

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Published on: September 24, 2012

Area of Science:

  • Proteomics
  • Biochemistry
  • Molecular Biology

Background:

  • Accurate quantification of protein complexes is crucial for understanding cellular mechanisms.
  • Existing methods often lack precision in determining the stoichiometry of protein interactions.

Purpose of the Study:

  • To present a novel mass spectrometry-based strategy for absolute quantification of protein complex components.
  • To enable precise measurement of interaction stoichiometries within protein networks.

Main Methods:

  • Utilized mass spectrometry for absolute quantification of proteins isolated via affinity purification.
  • Quantified bait proteins using isotope-labeled reference peptides of affinity tags.
  • Quantified prey proteins using label-free correlational quantification of proteotypic peptides from reciprocal purifications.

Main Results:

  • Successfully quantified protein complex components with high accuracy.
  • Determined interaction stoichiometries within the human protein phosphatase 2A network.
  • Demonstrated the efficacy of the developed mass spectrometry strategy.

Conclusions:

  • The presented mass spectrometry strategy provides a robust method for absolute protein complex quantification.
  • This technique is valuable for dissecting the stoichiometry of molecular interactions in biological systems.
  • The analysis of the protein phosphatase 2A network highlights the method's applicability.