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Related Experiment Video

Updated: Jun 25, 2026

An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

293 cell cycle synchronisation adenovirus vector production.

Tiago B Ferreira1, Ricardo Perdigão, Ana C Silva

  • 1ITQB-UNL/IBET, Apartado 12, P-2781-901 Oeiras, Portugal.

Biotechnology Progress
|February 10, 2009
PubMed
Summary
This summary is machine-generated.

Maximizing adenovirus vector (AdV) production requires optimizing cell density and cell cycle phase. Synchronizing 293 cells to the S phase significantly increases AdV titer, offering a scalable bioprocessing strategy.

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Area of Science:

  • Biotechnology
  • Virology
  • Cell Biology

Background:

  • Market demand for adenovirus vectors (AdV) necessitates increased virus titer per culture volume.
  • Traditional AdV production is limited by the "cell density effect," restricting cell density at infection.
  • Adenovirus vector production is influenced by the cell cycle phase during infection.

Purpose of the Study:

  • To investigate the impact of cell cycle synchronization on AdV production.
  • To determine optimal cell cycle phases for maximizing AdV titer.
  • To develop improved strategies for high-titer AdV manufacturing.

Main Methods:

  • 293 cells were chemically synchronized to various cell cycle phases.
  • Adenovirus vectors were produced following synchronization.
  • A temperature shift strategy (31°C to 37°C) was employed for S phase synchronization.
  • Mathematical modeling was used to correlate AdV productivity with S phase synchronization.

Main Results:

  • Chemical synchronization showed a 2.6-fold increase in AdV cell specific titer when S phase cells increased from 36% to 47%.
  • Temperature shift synchronization achieved 57% S phase population.
  • The temperature shift strategy resulted in a 7.3-fold increase in AdV cell specific titer.

Conclusions:

  • Adenovirus vector production is significantly enhanced by synchronizing cells to the S phase.
  • Temperature shift synchronization offers a non-chemical method to improve AdV yield.
  • Optimizing cell cycle phase is crucial for efficient AdV manufacturing.