Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intralipid adversely affects reticuloendothelial bacterial clearance.

S Katz1, B R Plaisier, W J Folkening

  • 1Department of Surgery, Indiana University School of Medicine, Indianapolis.

Journal of Pediatric Surgery
|August 1, 1991
PubMed
Summary

Intravenous fat emulsion administration may alter the reticuloendothelial system (RES) function, affecting bacterial clearance and organ localization. This study investigated the impact of fat emulsion administration routes on RES function and bacterial distribution in rats.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Duodenal duplication with bile duct obstruction and keratinaceous casts.

Pediatric surgery international·2013
Same author

Evaluation of perioperative cholangiography in one thousand laparoscopic cholecystectomies.

Chirurgia (Bucharest, Romania : 1990)·2006
Same author

Effects of interleukin-11 and epidermal growth factor on residual small intestine after experimental massive small bowel resection.

Chirurgia (Bucharest, Romania : 1990)·2006
Same author

Surgical techniques for pancreatic cancer.

Minerva chirurgica·2004
Same author

Cecal volvulus: a report of two cases occurring after the antegrade colonic enema procedure.

Journal of pediatric surgery·2004
Same author

Long-term analysis of children with esophageal atresia and tracheoesophageal fistula.

Journal of pediatric surgery·2003

Area of Science:

  • Immunology
  • Nutrition
  • Microbiology

Background:

  • Lipid emulsions are crucial for caloric intake in parenteral nutrition.
  • The reticuloendothelial system (RES), particularly Kupffer cells, clears lipid emulsions.
  • RES dysfunction can impair the ability to clear blood-borne bacteria.

Purpose of the Study:

  • To compare the effects of intraperitoneal (IP) versus intravenous (IV) fat emulsion (FE) administration on bacterial blood clearance and organ localization.
  • To evaluate the impact of FE administration timing on RES function.

Main Methods:

  • Rats received either IP or IV 20% fat emulsion or saline over three days.
  • Radiolabeled Escherichia coli were administered intravenously to assess bacterial clearance.
  • Blood and tissue samples (liver, spleen, kidney, lung) were analyzed for bacterial localization using liquid scintillation counting.

Related Experiment Videos

Main Results:

  • Rapid bacterial blood clearance occurred across all groups.
  • Intraperitoneal FE administration showed a slight but significant increase in bacterial localization in the lungs.
  • Organ distribution patterns of bacteria differed based on FE administration route and timing.

Conclusions:

  • Fat emulsion administration, particularly via the IP route, can influence bacterial localization in organs.
  • Further research is needed to fully understand the clinical implications of altered RES function due to parenteral nutrition lipids.