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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Related Experiment Video

Updated: Jun 25, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Recent developments in proteome informatics for mass spectrometry analysis.

James C Wright1, Simon J Hubbard

  • 1Faculty of Life Sciences, University of Manchester, Manchester M139PT, UK.

Combinatorial Chemistry & High Throughput Screening
|February 10, 2009
PubMed
Summary
This summary is machine-generated.

Proteomics bioinformatics tools are essential for analyzing complex mass spectrometry data. This review highlights recent advancements in proteome informatics for protein identification, quantitative proteomics, and data management.

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Last Updated: Jun 25, 2026

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Published on: April 11, 2019

Area of Science:

  • Proteomics
  • Bioinformatics
  • Analytical Chemistry

Background:

  • Mass spectrometry is the primary technique for studying proteins and proteomes.
  • High-throughput proteomic experiments generate vast amounts of complex data.
  • Bioinformatics is crucial for managing and interpreting this data.

Purpose of the Study:

  • To review recent developments in proteome informatics.
  • To discuss informatics tools for mass spectrometry-based proteomics.
  • To highlight challenges and future directions in the field.

Main Methods:

  • Review of recent literature on proteome informatics tools.
  • Discussion of tools for protein and peptide property prediction.
  • Overview of methods for peptide and protein identification from spectra.
  • Brief mention of informatics for quantitative proteomics.
  • Highlighting proteomic data repositories and standards.

Main Results:

  • Numerous informatics tools have been developed for proteomic data processing, analysis, storage, and integration.
  • New tools aid in predicting protein/peptide properties for experimental design.
  • Advancements facilitate peptide and protein identification from mass spectra.
  • Informatics approaches are emerging for quantitative proteomics.
  • Proteomic data repositories and standards are expanding.

Conclusions:

  • Proteome informatics is rapidly evolving to meet the challenges of mass spectrometry-based proteomics.
  • Emerging tools and technologies are paving the way for the next phase of proteomics research.
  • Continued development in bioinformatics is essential for future proteomics discoveries.