Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Induction of Pro-Fibrotic CLIC4 in Dermal Fibroblasts by TGF-β/Wnt3a Is Mediated by GLI2 Upregulation.

Cells·2022
Same author

Danger-associated molecular pattern molecules and the receptor for advanced glycation end products enhance ANCA-induced responses.

Rheumatology (Oxford, England)·2021
Same author

Bruton's tyrosine kinase regulates TLR7/8-induced TNF transcription via nuclear factor-κB recruitment.

Biochemical and biophysical research communications·2018
Same author

Transforming Growth Factor β Activation Primes Canonical Wnt Signaling Through Down-Regulation of Axin-2.

Arthritis & rheumatology (Hoboken, N.J.)·2018
Same author

The role of monocytes in ANCA-associated vasculitides.

Autoimmunity reviews·2016
Same author

Raised circulating tenascin-C in rheumatoid arthritis.

Arthritis research & therapy·2012

Related Experiment Video

Updated: Jun 25, 2026

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
12:50

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

Tyrosine kinases and inflammatory signalling.

Theresa H Page1, Maria Smolinska, Justin Gillespie

  • 1Kennedy Institute of Rheumatology, Imperial College of Science, Technology and Medicine, 65 Aspenlea Road, London W6 8LH, UK.

Current Molecular Medicine
|February 10, 2009
PubMed
Summary

Tyrosine kinases, including Src, Tec, and Syk families, are crucial in toll-like receptor (TLR) signaling and inflammation. Modulating these kinases offers therapeutic potential for chronic inflammatory diseases like rheumatoid arthritis, cardiovascular disease, and cancer.

More Related Videos

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells
07:12

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells

Published on: September 9, 2021

Related Experiment Videos

Last Updated: Jun 25, 2026

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
12:50

Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response

Published on: September 15, 2017

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells
07:12

Time-resolved Förster Resonance Energy Transfer Assays for Measurement of Endogenous Phosphorylated STAT Proteins in Human Cells

Published on: September 9, 2021

Area of Science:

  • Biochemistry
  • Immunology
  • Molecular Biology

Background:

  • Tyrosine kinases regulate critical cellular processes.
  • Their profound role in inflammation is increasingly recognized.
  • They are integral to toll-like receptor (TLR) signaling.

Purpose of the Study:

  • To review the role of tyrosine kinases in inflammation.
  • To emphasize their involvement in TLR signaling.
  • To highlight therapeutic strategies targeting tyrosine kinases.

Main Methods:

  • Literature review of tyrosine kinase involvement in inflammation.
  • Focus on Src, Tec, and Syk kinase families.
  • Analysis of their role in TLR signaling pathways.

Main Results:

  • Tyrosine kinases significantly influence gene expression, particularly cytokines.
  • They modulate both pro-inflammatory (e.g., TNF, IL-6) and anti-inflammatory (e.g., IL-10) cytokines.
  • Src, Tec, and Syk families are key mediators in TLR-induced inflammatory responses.

Conclusions:

  • Tyrosine kinases are central regulators of inflammatory responses via TLR signaling.
  • Targeting tyrosine kinases presents a promising therapeutic avenue for chronic inflammatory conditions.
  • Further research into kinase modulation could impact treatments for rheumatoid arthritis, cardiovascular disease, and cancer.