Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Types of Step-Growth Polymers: Polyesters

Types of Step-Growth Polymers: Polyesters

The introduction of polyesters has brought major development to the textile industry. The wrinkle-free behavior of polyester blends has eliminated the need for starching and ironing clothes.
Polyesters are commonly prepared from terephthalic acid and ethylene glycol; the crude product is known as poly(ethylene terephthalate) or PET. However, polyesters are synthesized industrially by transesterification of dimethyl terephthalate with ethylene glycol at 150 °C. The two reactants and the polymer...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Healing cascades and infections in wounds monitored using a wearable sensor of gaseous flux.

bioRxiv : the preprint server for biology·2026

Same author

Simultaneous regeneration of skin and bone in full-thickness cranial composite defects.

bioRxiv : the preprint server for biology·2026

Same author

Regulatory B cells contribute to allergen-encapsulating nanoparticle immunotherapy efficacy for food allergy.

JCI insight·2026

Same author

Immune Biomarkers of Islet Transplant Rejection Revealed by Synthetic Immunological Niche.

bioRxiv : the preprint server for biology·2026

Same author

Nanoparticle modulation of immune and vascular microenvironment dynamics following spinal cord injury.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Same author

Nanoparticle induction of antigen-presenting monocyte-derived dendritic cells relieves immunosuppression and inhibits metastasis.

Cell biomaterials·2026

Same journal

Cationic nanotrap curbs UVB-induced cutaneous photodamage via exosomal cfNA capture.

Biomaterials·2026

Same journal

A protein-initiated polymerization cascade enables a self-eliminating powder tissue adhesive for diabetic ulcer repair.

Biomaterials·2026

Same journal

Oral colon-targeted micro-nano formulation engineered in microfluid for synergistic therapy of inflammatory bowel disease.

Biomaterials·2026

Same journal

Manganese@Gold cluster-coordinated covalent organic frameworks-based artificial metalloenzymes with cascade biocatalysis and amplified systemic stimulation to combat malignant tumor metastasis.

Biomaterials·2026

Same journal

Remodeling TME via feedback-driven photothermal-ferroptosis-immune cascade.

Biomaterials·2026

Same journal

Corrigendum to "Photodynamic therapy produces enhanced efficacy of antitumor immunotherapy by simultaneously inducing intratumoral release of sorafenib" [Biomaterials 2020, 240, 119845].

Biomaterials·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Engineering Cell-permeable Protein

Engineering Cell-permeable Protein

Published on: December 28, 2009

Sustained transgene expression via citric acid-based polyester elastomers.

Xue-Qing Zhang¹, Huanghui Tang, Ryan Hoshi

¹Biomedical Engineering Department, Northwestern University, Evanston, IL 60208, USA.

|February 10, 2009

Summary

This summary is machine-generated.

Biodegradable polyester elastomer scaffolds effectively deliver genes for tissue engineering. Naked plasmid DNA on these scaffolds achieved long-term transgene expression in vivo, showing promise for non-viral gene therapy.

More Related Videos

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Published on: September 27, 2013

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Published on: May 7, 2018

Related Experiment Videos

Last Updated: Jun 25, 2026

Engineering Cell-permeable Protein

Engineering Cell-permeable Protein

Published on: December 28, 2009

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles

Published on: September 27, 2013

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Published on: May 7, 2018

Area of Science:

Biomaterials Science
Tissue Engineering
Gene Delivery

Background:

Porous polymeric scaffolds are crucial for tissue engineering and controlled gene delivery.
Biodegradable polyester elastomers offer potential as substrates for cell colonization and genetic material immobilization.

Purpose of the Study:

To investigate the use of poly(1,8-octanediol-co-citrate) (POC) scaffolds for plasmid DNA (pDNA) immobilization and subsequent cellular transfection.
To evaluate the efficacy of substrate-mediated gene delivery in vitro and in vivo.

Main Methods:

Surface immobilization of pDNA (naked or complexed with poly(ethyleneimine) (PEI) polyplexes) onto POC scaffolds via adsorption.
In vitro cell culture with HEK293 and porcine aortic smooth muscle cells (PASMC) to assess proliferation and transfection.
In vivo evaluation using a mouse intraperitoneal (IP) fat model to assess long-term transgene expression (luciferase and GFP).

Main Results:

Polyplex-loaded scaffolds exhibited higher pDNA loading and slower initial release rates compared to naked pDNA scaffolds.
In vitro studies showed sustained cell proliferation and transfection for up to 12 days on polyplex-loaded scaffolds.
In vivo studies demonstrated successful long-term transgene delivery (2 weeks) with naked pDNA-loaded POC scaffolds, achieving high luciferase and GFP expression.

Conclusions:

POC scaffolds are a suitable material for substrate-mediated gene delivery.
Non-viral gene delivery using naked pDNA immobilized on POC scaffolds can achieve long-term transgene expression in vivo.
POC scaffolds hold potential for supporting long-term biological cues to mediate tissue formation.