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Updated: Jun 25, 2026

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Published on: March 14, 2014

Stem cell characteristics in prostate cancer cell lines.

Minja J Pfeiffer¹, Jack A Schalken

¹Department of Urology, Radboud University Nijmegen Medical Centre, NL-6500HB Nijmegen, The Netherlands.

European Urology

|February 10, 2009

Summary

Stem-like cells may exist in prostate cancer cell lines, but CD133 is not a reliable marker for identifying them. Further research is needed to understand their role in tumor growth.

Area of Science:

Oncology
Cancer Stem Cell Biology

Background:

Recent research highlights the critical role of cancer stem-like cells in tumor repopulation.
Understanding these cells is key to developing effective cancer therapies.

Purpose of the Study:

To investigate the stem cell properties of six established prostate cancer (PCa) cell lines in vitro.
To evaluate potential stem cell markers, including CD133 and BCRP.

Main Methods:

Assessed colony-forming efficiency and self-renewal of prostate cancer cell lines using low-density plating and serial passaging.
Utilized flow cytometry and immunohistochemistry to analyze expression of CD133, BCRP, alpha2-integrin, nestin, CK5, and CK18.

Main Results:

Five of six PCa cell lines exhibited stem cell characteristics, forming distinct holoclones and paraclones.

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DU145 holoclones demonstrated self-renewal capacity.

A small fraction of CD133+ cells (0.01%) and BCRP+ cells (0.15%) were found in DU145 cells, but these did not show enhanced clonogenicity or holoclone formation.

Conclusions:

Established prostate cancer cell lines may harbor stem-like cell populations.
CD133 expression does not enrich for stem-like cells in these PCa models.
Alpha2-integrin and BCRP show potential as stem cell markers, while CK5/CK18 aid in distinguishing transient amplifying cells.