Related Experiment Video
Updated: Jun 25, 2026

Rodent Model of Masseter Volumetric Muscle Loss for Studying Bioengineering Materials
Published on: May 31, 2024
Effects on craniofacial growth and development of unilateral botulinum neurotoxin injection into the masseter muscle
Chi-Yang Tsai1, Wan Chi Chiu, Yi-Hsuan Liao
1Graduate Institute of Dentistry, Department of Orthodontics, College of Oral Medicine, Taipei Medical University and Hospital, Taipei, Taiwan. cytsai@tmu.edu.tw
Introduction:
The effects of botulinum neurotoxin type A (BoNT/A) on masseter muscles, when injected for cosmetic purposes (volumetric reduction) or treatment of excessive muscle activity (bruxism), have been investigated. However, the full anatomic effects of treatment are not known, particularly with respect to the mandible and relevant anthropometric measurements. The intent of this study was to use unilaterial BoNT/A injections to induce localized masseter atrophy and paresis and then to measure the effects of muscle influence on craniofacial growth and development.
Methods:
Growing male Wistar rats, 30 days old, were studied. The experimental group consisted of 8 rats. One side of the masseter muscle was injected with BoNT/A and the other side of the masseter muscle was injected with saline. The side with BoNT/A belonged to 1 group and the side with saline was the sham group. Three rats without injections was the control. After 45 days, the masseter muscles were dissected and weighed. Dry skulls were prepared, and anthropometric measurements determined.
Results:
One-way ANOVA showed that the animals maintained their weight in both groups; however, the muscles injected with BoNT/A were smaller than the sham or control muscles. Anthropometric measurements of the bony structures attached to the masseter muscle showed a significant treatment effect.
Conclusions:
After localized masseter muscle atrophy induced by BoNT/A injection, alterations of craniofacial bone growth and development were seen. The results agree with the functional matrix theory that soft tissues regulate bone growth.
Related Concept Videos
Skeletal Muscle Relaxants: Adverse Effects
Unlike...
Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin
The binding of dantrolene to the RYR1...
Muscles for Facial Expressions
Botulism
Nondepolarizing (Competitive) Neuromuscular Blockers: Pharmacological Actions
Although all competitive neuromuscular blockers are designed...
Skeletal Muscle Relaxants: Therapeutic Uses
