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Cell-type specific regulation of gene expression by simian virus 40 T antigens

Paul G Cantalupo1, Maria Teresa Sáenz-Robles, Abhilasha V Rathi

  • 1Department of Biological Sciences, 559 Crawford Hall, University of Pittsburgh Pittsburgh, Pennsylvania 15260, USA.

Virology
|February 10, 2009
PubMed

Insights

Simian virus 40 (SV40) alters ~800 genes via large T antigen, impacting cell growth. While some effects are universal, SV40 transformation shows cell-type specific gene regulation, influencing outcomes.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • SV40 oncoproteins (large T and small t antigens) drive cellular transformation.
  • Large T antigen affects cell proliferation, survival, and transcription factor binding.
  • Small t antigen interacts with phosphatase PP2A.

Purpose of the Study:

  • To investigate global gene expression changes induced by SV40 transformation.
  • To compare SV40-driven gene expression in mouse embryo fibroblasts (MEFs) and enterocytes.

Main Methods:

  • Analysis of global gene expression in SV40-transformed MEFs.
  • Examination of gene expression in enterocytes from SV40 transgenic mice.

Main Results:

  • SV40 transformation altered the expression of approximately 800 cellular genes in both MEFs and enterocytes.
  • A significant portion of gene regulation was consistent with the Rb-E2F pathway.
  • Cell-type specific gene regulation was observed, indicating unique signaling pathway activation.

Conclusions:

  • SV40 transformation induces widespread gene expression changes.
  • The Rb-E2F pathway is a common target of SV40 large T antigen.
  • SV40's impact on gene expression and cellular outcomes is context-dependent, varying by cell type.

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