Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
Hormones and Bone Tissue01:17

Hormones and Bone Tissue

The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Decoding distinctive features of plasma extracellular vesicles in amyotrophic lateral sclerosis.

Molecular neurodegeneration·2026
Same author

Temporal trends and spatial differences of trace elements in osprey eggs in Finland.

Environmental monitoring and assessment·2026
Same author

Calcium propionate in tortillas - a likely cause of a large outbreak of acute gastrointestinal illness, Finland, 2023.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2026
Same author

Activin-A has dual roles in osteoclast formation and foreign body giant cell differentiation from human CD14<sup>+</sup> monocytes.

Bone·2026
Same author

Regenerative potential of nanoenabled collagen-polylactide scaffolds for osteochondral defect repair in rabbits.

Frontiers in bioengineering and biotechnology·2025
Same author

Treatment with the Bifidobacterium longum Strain DSM 32947 Increases Bone Mineral Density in Female Mice.

Calcified tissue international·2025

Related Experiment Video

Updated: Jun 25, 2026

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

Dioxin-sensitive proteins in differentiating osteoblasts: effects on bone formation in vitro.

Donatella Carpi1, Merja Korkalainen, Luisa Airoldi

  • 1Department of Environmental Health Sciences, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.

Toxicological Sciences : an Official Journal of the Society of Toxicology
|February 10, 2009
PubMed
Summary

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), an endocrine disruptor, alters bone development by affecting osteoblast differentiation. This study identifies key proteins and pathways involved in TCDD

More Related Videos

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
11:11

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Published on: May 19, 2019

Related Experiment Videos

Last Updated: Jun 25, 2026

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
11:11

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Published on: May 19, 2019

Area of Science:

  • Environmental Toxicology
  • Molecular Biology
  • Bone Biology

Background:

  • 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is an endocrine-disrupting environmental pollutant with known effects on bone tissue.
  • The precise molecular mechanisms by which TCDD impacts osteogenesis remain unclear.

Purpose of the Study:

  • To investigate the effects of TCDD on osteoblast differentiation using a proteomic approach.
  • To identify specific proteins and pathways affected by TCDD during osteogenesis in vitro.

Main Methods:

  • Utilized an in vitro osteoblast differentiation model using rat mesenchymal stem cells.
  • Employed a proteome approach to analyze protein abundance changes after TCDD exposure.
  • Confirmed cellular alterations using confocal microscopy and analyzed pathway involvement with MetaCore.

Main Results:

  • Significant changes in the abundance of 18 proteins were observed after 10 days of TCDD exposure.
  • Affected proteins were primarily involved in cytoskeleton organization, actin-based processes, and protein transport.
  • TCDD exposure led to altered cell architecture, increased cell adhesion, and reduced calcium deposition.
  • A decrease in calcium-binding proteins was noted, potentially impacting osteoblast function.

Conclusions:

  • This study provides the first proteomic insights into TCDD's effects on osteoblastic differentiation.
  • Identified proteins and pathways, particularly those linked to c-fos and c-myc, suggest novel mechanisms for TCDD-induced inhibition of osteogenesis.
  • Findings reveal new molecular players contributing to TCDD's impact on bone development.