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Updated: Jun 25, 2026

Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System
Published on: October 18, 2018
Treatment of fungal bioaerosols by a high-temperature, short-time process in a continuous-flow system
Jae Hee Jung1, Jung Eun Lee, Chang Ho Lee
1Center for Environmental Technology Research, Korea Institute of Science and Technology, Hawolgok-dong, Seongbuk-gu, Seoul 136-791, Republic of Korea.
Abstract:
Airborne fungi, termed fungal bioaerosols, have received attention due to the association with public health problems and the effects on living organisms in nature. There are growing concerns that fungal bioaerosols are relevant to the occurrence of allergies, opportunistic diseases in hospitals, and outbreaks of plant diseases. The search for ways of preventing and curing the harmful effects of fungal bioaerosols has created a high demand for the study and development of an efficient method of controlling bioaerosols. However, almost all modern microbiological studies and theories have focused on microorganisms in liquid and solid phases. We investigated the thermal heating effects on fungal bioaerosols in a continuous-flow environment. Although the thermal heating process has long been a traditional method of controlling microorganisms, the effect of a continuous high-temperature, short-time (HTST) process on airborne microorganisms has not been quantitatively investigated in terms of various aerosol properties. Our experimental results show that the geometric mean diameter of the tested fungal bioaerosols decreased when they were exposed to increases in the surrounding temperature. The HTST process produced a significant decline in the (1-->3)-beta-d-glucan concentration of fungal bioaerosols. More than 99% of the Aspergillus versicolor and Cladosporium cladosporioides bioaerosols lost their culturability in about 0.2 s when the surrounding temperature exceeded 350 degrees C and 400 degrees C, respectively. The instantaneous exposure to high temperature significantly changed the surface morphology of the fungal bioaerosols.
Insights
High-temperature, short-time (HTST) heating effectively controls airborne fungi (fungal bioaerosols). This method reduces fungal particle size and (1-->3)-beta-d-glucan levels, inactivating over 99% of common airborne fungi.
Area of Science:
- Environmental microbiology
- Aerosol science
- Public health
Background:
- Fungal bioaerosols pose risks to public health, causing allergies and opportunistic infections.
- Concerns are rising regarding fungal bioaerosols' impact on allergies, hospital-acquired diseases, and plant pathology.
- Current research predominantly focuses on microorganisms in liquid and solid phases, neglecting airborne forms.
Purpose of the Study:
- To investigate the effects of thermal heating on fungal bioaerosols in a continuous-flow system.
- To quantitatively assess the impact of high-temperature, short-time (HTST) processing on airborne fungal properties.
- To explore novel methods for controlling harmful fungal bioaerosols.
Main Methods:
- Experimental investigation of thermal heating effects on fungal bioaerosols.
- Utilizing a continuous-flow environment for exposure.
- Measuring changes in aerosol properties, including geometric mean diameter and (1-->3)-beta-d-glucan concentration.
Main Results:
- Geometric mean diameter of fungal bioaerosols decreased with increasing temperature.
- HTST processing significantly reduced (1-->3)-beta-d-glucan concentration.
- Over 99% inactivation of Aspergillus versicolor and Cladosporium cladosporioides occurred at temperatures exceeding 350°C and 400°C, respectively, within 0.2 seconds.
- High-temperature exposure altered the surface morphology of fungal bioaerosols.
Conclusions:
- HTST heating is a promising method for controlling airborne fungal bioaerosols.
- The study provides quantitative data on the efficacy of thermal inactivation for specific fungal species.
- Thermal processing significantly impacts fungal bioaerosol characteristics and viability.
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