Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pig kidney phosphofructokinase. Purification to homogeneity and qualitative basic characterization.

N Q Khang, H J Böhme, E Hofmann

    Acta Biologica Et Medica Germanica
    |January 1, 1976
    PubMed
    Summary

    This study details the purification of pig kidney phosphofructokinase, revealing its enzymatic properties and subunit molecular weight. The purified enzyme shows sigmoidal kinetics and is regulated by ATP, citrate, AMP, cAMP, and ADP.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Management of Invasive Pulmonary Aspergillosis in Intensive Care Units: Guidelines From the Fungal Infection Network of Switzerland (FUNGINOS).

    Mycoses·2025
    Same author

    Time Trends in Causes of Death in People With HIV: Insights From the Swiss HIV Cohort Study.

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2024
    Same author

    Continuous pH monitoring in wounds using a composite indicator dressing - A feasibility study.

    Burns : journal of the International Society for Burn Injuries·2019
    Same author

    A structural view of synthetic cofactor integration into [FeFe]-hydrogenases.

    Chemical science·2018
    Same author

    Chalcogenide substitution in the [2Fe] cluster of [FeFe]-hydrogenases conserves high enzymatic activity.

    Dalton transactions (Cambridge, England : 2003)·2017
    Same author

    Interpersonal stroking touch is targeted to C tactile afferent activation.

    Behavioural brain research·2015

    Area of Science:

    • Biochemistry
    • Enzymology

    Background:

    • Phosphofructokinase (PFK) is a key regulatory enzyme in glycolysis.
    • Understanding PFK from different sources, like pig kidney, is crucial for metabolic studies.

    Purpose of the Study:

    • To purify phosphofructokinase from pig kidney.
    • To characterize its enzymatic properties and subunit molecular weight.

    Main Methods:

    • Purification involved phosphate buffer extraction, alcohol treatment, affinity chromatography (Cibacron blue F3G-A), and gel chromatography (Sepharose 6B).
    • Enzyme homogeneity and subunit molecular weight were assessed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE).
    • Enzyme kinetics and regulatory mechanisms were studied through substrate concentration-response curves and inhibitor/activator assays.

    Related Experiment Videos

    Main Results:

    • Homogeneous phosphofructokinase was obtained with a specific activity of approximately 80 units/mg protein.
    • The enzyme exhibited sigmoidal kinetics with respect to fructose 6-phosphate concentration at pH 7.0.
    • Strong inhibition by ATP was observed, which was modulated by citrate, fructose 6-phosphate, AMP, cAMP, and ADP.

    Conclusions:

    • Pig kidney phosphofructokinase was successfully purified and characterized.
    • The enzyme's kinetic and regulatory properties are consistent with its role in glycolysis control.
    • The subunit molecular weight was determined to be 88,000 Da.