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Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors
Published on: November 24, 2010
Linking dengue virus entry and translation/replication through amphisomes
Mingkwan Panyasrivanit1, Atefeh Khakpoor, Nitwara Wikan
1Molecular Pathology Laboratory, Institute of Molecular Biology and Genetics, Mahidol University, Nakorn Pathom, Thailand.
Abstract:
Amphisomes are preautolysosomal vacuoles formed upon the fusion of autophagosomes with endosomes, and as such represent a critical meeting point between endocytic and autophagic pathways. Dengue virus enters into susceptible cells by clathrin-mediated endocytosis, and colocalization of dengue markers with markers of both autophagic and endosomal vesicles demonstrates that amphisomes are a site of dengue virus replication and translation. This work links for the first time the processes of dengue virus entry and translation/replication, and allows for interpretation of the early part of the dengue virus life cycle in terms of a continual association with membranes of an endosomal-autophagosomal lineage.
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