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Updated: Jun 25, 2026

Extraction of Saliva, Haemolymph, Salivary Glands, and Midgut from Individual Ticks (Acari: Ixodidae)
Published on: October 31, 2025
Some hydrolase activities from the tick Hyalomma lusitanicum koch, 1844 (Ixodoidea: Ixodida)
C Giménez-Pardo1, M M Martínez-Grueiro
1Departamento de Microbiología y Parasitología, Universidad de Alcalá, Ctra. Madrid-Barcelona, Km 33.6, 28871 Alcalá de Henares, Madrid, Spain. consuelo.gimenez@uah.es
Abstract:
In this work has been made a detection and preliminary characterization of some hydrolases in whole extracts from unfed adult males and females of Hyalomma lusitanicum, one of the vectors for Theileria annulata that causes Mediterranean theileriosis in cattle. We have elected as targets, proteases as enzymes implicated in the nutritional processes of ticks, esterases that are usually implicated in resistance to organophosphates and phosphatises often implicated in protein phosphorilation and control of ticks salivary gland. The biological role and physiological significance are discussed in terms of the possibility of use these enzymes as possible in future anti-tick vaccination or acaricide resistance.

