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Published on: May 1, 2019
Purification of nine sulfonamides from chicken tissues by immunoaffinity chromatography using two monoclonal
Bingyu Li1, Cun Li, Haiyang Jiang
1China Agricultural University, National Reference Laboratory for Veterinary Drug Residue, Department of Pharmacology and Toxicology, College of Veterinary Medicine, Beijing 100094, People's Republic of China.
Abstract:
An immunoaffinity chromatographic method was developed for the purification of 9 sulfonamides (sulfamethazine, sulfadimethoxine, sulfamerazine, sulfathiazole, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamonomethoxine, and sulfapyridine) from chicken tissue (muscle and liver) samples. Two monoclonal antibodies (antisulfamethazine and antisulfamethoxazole) were simultaneously covalently coupled to CNBr-activated Sepharose 4B for the preparation of a re-usable immunoaffinity column. After extraction with methanol-water and purification by immunoaffinity column, the sulfonamides were determined by reversed-phase liquid chromatography and UV detection at 270 nm. The recoveries for each drug at fortification levels of 10-50 ng/g ranged from 74.1 to 108.9% with relative standard deviations from 1.9 to 11.5%. The limits of detection were 2 ng/g for each drug.
