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Development of enucleated mouse oocytes reconstituted with embryonic nuclei
1NODAI Research Institute, Tokyo University of Agriculture, Japan.
Journal of Reproduction and Fertility
|September 1, 1991
Summary
Nuclear transplantation in mouse oocytes enables development. Enucleated oocytes receiving nuclei from early embryos can develop into blastocysts and produce live offspring, demonstrating potential for developmental biology research.
Area of Science:
- Developmental Biology
- Reproductive Science
- Cell Biology
Background:
- Oocyte maturation is crucial for female meiosis.
- Nuclear transplantation is a key technique in reproductive biology.
Purpose of the Study:
- To investigate the developmental potential of enucleated mouse oocytes after nuclear transplantation.
- To assess the viability of reconstituted embryos derived from oocytes and various embryonic nuclei.
Main Methods:
- Micromanipulation and differential interference microscopy were used to remove chromosomes from mouse oocytes.
- Enucleated oocytes served as recipients for nuclear transplantation.
- Reconstituted embryos were cultured and assessed for blastocyst development.
Main Results:
- Newly synthesized proteins in enucleated oocytes mimicked those of in vivo matured secondary oocytes.
- Nuclear transplantation with nuclei from 2-cell and 8-cell embryos, and inner cell mass cells resulted in 23%, 4%, and 10% blastocyst development, respectively.
- Live young were produced from reconstituted embryos receiving nuclei from late 2-cell stage embryos.
Conclusions:
- Enucleated mouse oocytes can support embryonic development after nuclear transplantation.
- The developmental potential of reconstituted embryos depends on the origin of the transplanted nucleus.
- This study highlights the efficiency of nuclear transfer in early mouse embryos for producing viable offspring.