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Updated: Jun 25, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
Published on: January 16, 2017
Enantioseparation of binaphthol and its monoderivatives by cyclodextrin-modified capillary zone electrophoresis: a
N Mofaddel1, H Krajian, D Villemin
1L.A.S.O.C. - IRCOF et IFRMP, Université de Rouen, 55 rue Saint Germain, 27000 Evreux, France.
Abstract:
A simple model for the separation of atropisomers of binaphthol and its monoderivatives by means of cyclodextrin-modified capillary zone electrophoresis (CD-CZE) was used to describe the migration behavior of poly charged enantiomers in a chiral separation system. This mathematical approach allowed for the determination of the optimal cyclodextrin concentrations for the enantioseparation of binaphthols by taking into account the influence of the formed complex mobilities. Moreover, using this theoretical approach, the reversal of the enantiomers' migration order as a function of cyclodextrin concentration was predicated. The apparent complexation constants between the cyclodextrins and the binaphthol and its monoderivatives could be calculated using a non-linear curve fitting method and three linear plotting methods (x-reciprocal, y-reciprocal and double reciprocal). Good agreements between the theoretical and experimental cyclodextrin concentrations were obtained.
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