HPLC analysis of organic acids using a novel stationary phase

In the present work, a high performance liquid chromatographic method with UV detection for the separation of six organic acids including, tartaric, malic, acetic, lactic, citric and succinic is described. The separation was performed on a novel stationary phase TEKNOKROMA, Tr-010065 Mediterranea sea(18) (15 cm x 0.4 cm, i.d. 3 microm) and using water with a 0.1% (v/v) of formic acid as mobile phase. The advantages of this packing over a conventional octadecylsilane (ODS2) column are reported. The method was validated with respect to linearity, limits of detection and repeatabilities within day and between days and satisfactory results were obtained. The proposed method was applied for the determination of these compounds in commercially available white wines. The samples were injected directly without previous treatment. LC-MS was used as a confirmatory technique.