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Updated: Jun 25, 2026

Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device
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Monolithic microextraction tips by emulsion photopolymerization.

Shih-Shin Liang1, Shu-Hui Chen

  • 1Department of Chemistry, National Cheng Kung University, Tainan, Taiwan.

Journal of Chromatography. A
|February 11, 2009
PubMed
Summary
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New emulsion techniques create large-pore monoliths for pipette tip microextraction. This method reduces back pressure and enhances sample preparation efficiency for diverse applications.

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Microfluidics

Background:

  • Photopolymerized monoliths are used in microdevices for sample preparation.
  • Existing methods create high back pressure in microextraction tips.
  • Need for improved monoliths with low back pressure and high permeability.

Purpose of the Study:

  • To develop monoliths with large through pores (>20 microm) using emulsion techniques.
  • To fabricate rigid monoliths on pipette tips for microextraction.
  • To demonstrate the encapsulation of functionalized particles for solid-phase microextraction.

Main Methods:

  • Utilized oil-in-water emulsion techniques for monolith fabrication.
  • Employed photopolymerization to create rigid structures on pipette tips.

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  • Incorporated functionalized particles (5-20 microm) into stable emulsions for encapsulation.

    • Main Results:

    • Achieved monoliths with large through pores (>20 microm) and rigid structures.
    • Demonstrated successful encapsulation of various functionalized particles.
    • Obtained high recovery rates (90-109%) for desalting and phosphopeptide enrichment.
    • Showcased linear calibration curves for estrogenic compounds (R(2)>0.99).

    Conclusions:

    • Emulsion techniques enable the fabrication of low-back-pressure, high-permeability monoliths for microextraction.
    • Functionalized monoliths are effective for qualitative and quantitative solid-phase microextractions.
    • This approach offers a versatile platform for miniaturized sample preparation.