Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Toward the Synthesis of Pestalustaine A: Structural Revision and Formation of Original Strained Tricyclic Architectures.

Angewandte Chemie (International ed. in English)·2026

Same author

Proposing an identity-in-context framework for culturally responsive evaluation.

Evaluation and program planning·2026

Same author

ReO<sub>x</sub>/TiO<sub>2</sub>: An Efficient Catalyst for the Production of Butanediols From Erythritol in Water.

ChemSusChem·2026

Same author

Radiology Program Director Wellness, Value of the use of Peer Networks.

Academic radiology·2026

Same author

Total Synthesis of the Spirocyclic Bis-Indole Alkaloid (-)-Owerreine via a [4+2] Annulation.

Angewandte Chemie (International ed. in English)·2026

Same author

Potassium Bisulfite's Role in Developing a Robust Platform for Enantioenriched <i>N</i>-Alkylpyridinium Salts as Piperidine Precursors.

Journal of the American Chemical Society·2026

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

Total synthesis of rapamycin.

Steven V Ley¹, Miles N Tackett, Matthew L Maddess

¹Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK. svl1000@cam.ac.uk

Chemistry (Weinheim an Der Bergstrasse, Germany)

|February 11, 2009

Summary

Researchers developed a novel, convergent synthesis for rapamycin, a potent immunosuppressant and anti-cancer drug. This new method utilizes macro-etherification and catechol-templating to efficiently construct the complex macrocyclic core.

More Related Videos

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Published on: September 6, 2024

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

Related Experiment Videos

Last Updated: Jun 25, 2026

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Published on: September 6, 2024

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

Area of Science:

Natural Product Synthesis
Medicinal Chemistry
Organic Synthesis

Background:

Rapamycin is a natural product with significant immunosuppressive and anti-cancer properties.
Its complex macrocyclic structure presents a substantial synthetic challenge.
Rapamycin and its derivatives are increasingly investigated for various human malignancies.

Purpose of the Study:

To detail a new and convergent synthetic strategy for rapamycin.
To overcome the synthetic challenges associated with rapamycin's macrocyclic core.
To provide a robust method for accessing rapamycin and its analogs.

Main Methods:

Development of a convergent synthetic route.
Implementation of a macro-etherification strategy.
Utilization of catechol-templating for macrocycle construction.

Main Results:

Successful synthesis of rapamycin via a novel convergent approach.
Efficient construction of the macrocyclic core using the developed strategy.
Demonstration of a new method for natural product synthesis.

Conclusions:

The new synthetic strategy offers an efficient and convergent pathway to rapamycin.
This approach facilitates the synthesis of rapamycin and its derivatives for therapeutic applications.
The macro-etherification/catechol-templating strategy is a powerful tool for complex natural product synthesis.