Related Experiment Video

Updated: Jun 25, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

[Various effects of serine proteinases, activated protein C and duodenase, on mast cells]

A M Makarova, L R Gorbacheva, T S Zamolodchikova

    Biomeditsinskaia Khimiia
    |February 12, 2009
    PubMed

    Some serine proteinases of haemostasis can regulate blood clotting and inflammation acting at proteinase-activated receptors (PARs). It is known that the anticoagulant proteinase, activated protein C (APC), exhibits anti-inflammatory effects on endothelial cells and macrophages and this involves endothelial protein C receptor--EPCR and proteinase-activated receptor--PAR1. We have studied the effect of wide range of APC concentrations on functional activity of rat peritoneal mast cells (PMC), which secrete the proinflammatory mediators, under normal conditions and during acute inflammation in rats. APC was able to reduce beta-hexosaminidase release from PMC. APC at very low concentrations (0.2-2 nM) modulated the mediator secretion from PMC under normal conditions and also during acute inflammation in rats. APC abolished the proinflammatory activity of duodenase (80 nM), the proteinase from gastrointestinal tract and mast cells. Mast cells pretreated with cathepsin G (PAR1 antagonist) or duodenase abolished protective antiinflammatory effect of low concentrations of APC on PMC degranulation. Our data indicated that blockade of the mast cells proinflammatory mediator secretion by APC involved PAR1 activation.

    More Related Videos

    Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice09:07

    Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice

    Published on: May 27, 2015

    Related Concept Videos

    Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs01:25

    Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs

    Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
    Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
    Role of Matrix Metalloproteases in Degradation of ECM01:23

    Role of Matrix Metalloproteases in Degradation of ECM

    Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
    A...
    cAMP-dependent Protein Kinase Pathways01:25

    cAMP-dependent Protein Kinase Pathways

    Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
    Allergic Reactions: Anaphylaxis01:30

    Allergic Reactions: Anaphylaxis

    Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin, heparin),...
    GPCRs Regulate Adenylyl Cylase Activity01:09

    GPCRs Regulate Adenylyl Cylase Activity

    Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of cells.
    Two...
    Allergic Reactions02:06

    Allergic Reactions

    Overview