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Infectious entry pathway of adenovirus type 2

M J Varga1, C Weibull, E Everitt

  • 1Department of Microbiology, University of Lund, Sweden.

Journal of Virology
|November 1, 1991
PubMed

Insights

Human adenovirus type 2 enters HeLa cells via receptor-mediated endocytosis, utilizing the coated-pit and -vesicle pathway. This process is crucial for initiating the virus's infectious cycle.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Human adenovirus type 2 (HAdV2) is a significant human pathogen.
  • Understanding viral entry mechanisms is crucial for developing antiviral strategies.

Purpose of the Study:

  • To investigate the internalization pathway of infectious human adenovirus type 2 into HeLa cells.
  • To determine if adenovirus entry utilizes receptor-mediated endocytosis.

Main Methods:

  • Quantitative internalization assay using infectious HAdV2 and HeLa cells.
  • Treatment with agents known to block receptor-mediated endocytosis.
  • Comparison of HAdV2 uptake with transferrin endocytosis.

Main Results:

  • Treatments blocking receptor-mediated endocytosis significantly reduced HAdV2 internalization.
  • The reduction in viral uptake mirrored the reduction in transferrin endocytosis.
  • This indicates HAdV2 utilizes the same pathway as transferrin for entry.

Conclusions:

  • Internalization of infectious human adenovirus type 2 into HeLa cells occurs via the coated-pit and -vesicle pathway.
  • This receptor-mediated endocytosis is an early and essential step in the HAdV2 infectious cycle.

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