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Detection of Protease Activity by Fluorescent Peptide Zymography

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

Peptide-modified optical filters for detecting protease activity.

Kristopher A Kilian¹, Till Böcking, Katharina Gaus

¹School of Chemistry, Centre for Vascular Research, University of New South Wales, Sydney NSW 2052, Australia.

|February 12, 2009

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This study presents a novel method for detecting protease enzymes using peptide-functionalized silicon photonic crystals. The developed biosensor achieved sensitive detection of subtilisin, a protease, down to 37 nM concentrations.

Area of Science:

Nanotechnology
Biochemistry
Materials Science

Background:

Nanoporous photonic crystals offer sensitive detection platforms due to their optical properties.
Immobilizing biomolecules on these surfaces is crucial for biosensor development.
Protease enzyme detection is vital in various biological and medical applications.

Purpose of the Study:

To develop a method for immobilizing peptides onto silicon-based nanoporous photonic crystals.
To create a sensitive biosensor for detecting protease enzymes in solution.
To characterize the functionalization process and assess the sensor's detection limits.

Main Methods:

Fabrication of a one-dimensional silicon photonic crystal (rugate filter).
Organic derivatization of the silicon surface via hydrosilylation and subsequent modifications with alkene and ethylene glycol derivatives.

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Last Updated: Jun 25, 2026

Detection of Protease Activity by Fluorescent Peptide Zymography

Detection of Protease Activity by Fluorescent Peptide Zymography

Published on: January 20, 2019

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

Published on: August 4, 2009

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Activation of the surface for peptide immobilization using disuccinimidyl carbonate.

Detection of protease activity by monitoring shifts in the photonic crystal's optical resonance.

X-ray photoelectron spectroscopy (XPS) for surface analysis.

Main Results:

Successful immobilization of peptides onto the functionalized photonic crystal surface.
Demonstrated sensitivity of the photonic crystal's optical resonance to refractive index changes caused by peptide cleavage.
Achieved a lowest detected concentration of 37 nM for the protease subtilisin.
XPS confirmed high efficiency in surface activation and coupling steps.

Conclusions:

Organic derivatization of silicon photonic crystals provides an effective platform for peptide immobilization.
The developed biosensor demonstrates high sensitivity and specificity for protease detection.
This approach holds promise for developing advanced optical biosensors for enzyme analysis in complex samples.