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Published on: February 14, 2018
Optimization of bioassay method for the quantitative microbiological determination of amphotericin B
András Fittler1, Béla Kocsis, Imre Gerlinger
1Department of Pharmaceutics and University Pharmacy, University of Pécs, Pécs, Hungary. andras.fittler@aok.pte.hu
Abstract:
The aim of this study was to find the optimal bioassay parameters for the quantitative analysis of an amphotericin B nasal spray solution as the bioassay conditions recommended by the Ph. Eur. 6. were less sensitive and were only applicable for the measurement of a narrow concentration range, which makes the method unsuitable in case of a stability test. We evaluated five commonly used assay media with Candida albicans and Saccharomyces cerevisiae as test organisms. Our results showed that Mueller Hinton Agar supplemented with 2% glucose and 0.5 microg ml(-1) methylene blue inoculated with C. albicans gave the best bioassay circumstance as a wide concentration range (1.54-60.0 microg ml(-1) amphotericin B) could be measured and the inhibition zone borders were distinct and easy to read.
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