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Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
IP3/DAG Signaling Pathway01:11

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Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
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Receptor-mediated Endocytosis01:20

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Amplifying Signals via Second Messengers01:15

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Colonisation of Pathogens01:25

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Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...

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Updated: Jun 25, 2026

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Published on: July 26, 2019

Pathogen trafficking pathways and host phosphoinositide metabolism.

Stefan S Weber1, Curdin Ragaz, Hubert Hilbi

  • 1Institute of Microbiology, ETH Zürich, Wolfgang-Pauli-Strasse 10, 8093 Zürich, Switzerland.

Molecular Microbiology
|February 12, 2009
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Summary

Intracellular bacterial pathogens hijack host phosphoinositide (PI) metabolism to control cell entry and replication. Pathogens manipulate PI levels to establish distinct intracellular niches, highlighting PI

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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Published on: January 6, 2016

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
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A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

Area of Science:

  • Cell Biology
  • Microbiology
  • Biochemistry

Background:

  • Phosphoinositide (PI) glycerolipids are crucial for eukaryotic cell signaling, cytoskeleton organization, and membrane trafficking.
  • Intracellular bacterial pathogens exploit host cell PI metabolism for survival and replication within host cells.

Purpose of the Study:

  • To elucidate the intricate strategies employed by intracellular bacterial pathogens to manipulate host phosphoinositide metabolism.
  • To understand how pathogens utilize PI metabolism to modulate host cell entry, vesicle trafficking, and establish replicative niches.

Main Methods:

  • Analysis of effector proteins translocated by vacuolar pathogens (e.g., Legionella pneumophila, Salmonella enterica) that interact with PIs.
  • Investigation of cytoplasmic pathogens' strategies targeting plasma membrane PI metabolism for uptake and survival.
  • Examination of pathogen-host interactions involving PI modification or binding to PI-associated host factors.

Main Results:

  • Vacuolar pathogens modify host PI levels to mimic or alter subcellular membranes, creating specialized replicative compartments.
  • Cytoplasmic pathogens target plasma membrane PI metabolism to influence invasion and evade apoptosis.
  • Specific bacterial effectors directly modify PIs or indirectly exploit PI-dependent host pathways.

Conclusions:

  • Host phosphoinositide metabolism is a critical target for a wide range of intracellular bacterial pathogens.
  • Pathogen strategies for manipulating PI metabolism are diverse, adapting to either vacuolar or cytoplasmic lifestyles.
  • Understanding these interactions provides insights into host-pathogen dynamics and potential therapeutic targets.