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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

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Related Experiment Video

Updated: Jun 25, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Cellular stress and RNA splicing.

Giuseppe Biamonti1, Javier F Caceres

  • 1Istituto di Genetica Molecolare-Consiglio Nazionale delle Ricerche Via Abbiategrasso 207, 27100 Pavia, Italy. biamonti@igm.cnr.it

Trends in Biochemical Sciences
|February 12, 2009
PubMed
Summary
This summary is machine-generated.

Cells activate survival strategies under stress, including inhibiting pre-messenger RNA (pre-mRNA) splicing. Different mechanisms block constitutive and alternative splicing, impacting gene expression during stress recovery.

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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Last Updated: Jun 25, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Area of Science:

  • Molecular Biology
  • Cellular Stress Response
  • Gene Expression Regulation

Background:

  • Cells activate gene expression for survival against physical and chemical stresses that disrupt protein folding and metabolism.
  • Pre-messenger RNA (pre-mRNA) splicing is a key cellular process targeted by various stress agents, notably heat shock.
  • Recent research is elucidating the molecular mechanisms behind stress-induced splicing inhibition.

Purpose of the Study:

  • To investigate how cellular stresses, particularly heat shock, impact pre-mRNA splicing.
  • To differentiate the molecular mechanisms affecting constitutive and alternative pre-mRNA splicing under stress.
  • To understand the role of splicing modulation in cellular adaptation and recovery from stress.

Main Methods:

  • Analysis of gene expression patterns under various stress conditions.
  • Molecular assays to examine the inhibition of constitutive splicing.
  • Investigating regulatory mechanisms affecting alternative splicing during stress response.

Main Results:

  • Heat shock and other stresses inhibit pre-mRNA splicing through distinct molecular pathways.
  • Constitutive pre-mRNA splicing is blocked by specific stress-induced mechanisms.
  • Alternative splicing regulation is also affected, potentially allowing for adaptive gene expression changes.

Conclusions:

  • Pre-mRNA splicing is a critical nexus for integrating cellular and metabolic stress signals into gene expression.
  • Differential regulation of splicing pathways under stress is crucial for cellular survival and recovery.
  • Understanding splicing inhibition provides insights into cellular stress response and adaptation.