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A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Published on: March 15, 2017
A high performance liquid chromatography method for simultaneous determination of rosiglitazone and gemfibrozil in
Xin Kang1, Feng Wang, Zhihong Xie
1The Clinical Pharmacy and Pharmacology Institute, Second Xiangya Hospital, Central South University, Changsha 410011, PR China. kangx08@163.com
Abstract:
A high performance liquid chromatography (HPLC) method was developed for the simultaneous determination of rosiglitazone and gemfibrozil in human plasma using alpha-asarone as an internal standard (IS). Plasma samples were pretreated by protein precipitation. The analytes were separated on a Macherey-Nagel Nucleodur C(18) (250 mm x 4.6 mm, 5 microm) column using acetonitrile and 30 mmol/l ammonium acetate solution (including 0.1% methanoic acid) as mobile phase which was delivered at 1.2 ml/min. A gradient elution program was adopted to adjust proportion of solvent in mobile phase. A time program was used to regulate conditions of fluorescence detection. The method was validated over the concentration range of 5.0-751.3 ng/ml for rosiglitazone and 0.5-75.4 microg/ml for gemfibrozil with acceptable accuracy, precision and extraction recoveries. This method is suitable for routine therapeutic drug monitoring and pharmacokinetic interaction study of the two drugs.
