Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Postoperative wound care protocol prevents surgical site infection after craniotomy.

Infection control and hospital epidemiology·2024

Same author

MHC heterozygosity limits T cell receptor variability in CD4 T cells.

Science immunology·2024

Same author

Aire mediates tolerance to insulin through thymic trimming of high-affinity T cell clones.

Proceedings of the National Academy of Sciences of the United States of America·2024

Same author

Improving Nurse-Physician Bedside Communication Using a Patient Experience Quality Improvement Pilot Project at an Academic Medical Center.

Cureus·2024

Same author

Vaccine-elicited B- and T-cell immunity to SARS-CoV-2 is impaired in chronic lung disease patients.

ERJ open research·2023

Same author

Vaccine-elicited B and T cell immunity to SARS-CoV-2 is impaired in chronic lung disease patients.

medRxiv : the preprint server for health sciences·2023

Search research articles

Related Experiment Video

Updated: Jun 25, 2026

Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

Published on: May 12, 2023

Transgenic Bcl-3 slows T cell proliferation.

Michael F J Bassetti¹, Janice White, John W Kappler

¹Integrated Department of Immunology, University of Colorado Denver Health Sciences Center, Denver, CO 80206, USA.

International Immunology

|February 12, 2009

Summary

This summary is machine-generated.

Immunological adjuvants boost T cell survival by increasing Bcl-3, a transcriptional regulator. Overexpression of Bcl-3 independently slows T cell activation, suggesting a complex role in immune responses.

More Related Videos

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Published on: December 27, 2016

Related Experiment Videos

Last Updated: Jun 25, 2026

Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

Published on: May 12, 2023

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Published on: December 27, 2016

Area of Science:

Immunology
Molecular Biology
Cell Biology

Background:

Immunological adjuvants, like bacterial lipopolysaccharide (LPS), enhance immune responses.
Adjuvants increase messenger RNA (mRNA) levels of Bcl-3, a transcriptional activator, in activated T cells.
Bcl-3 overexpression and adjuvants both extend T cell lifespan, implying a connection.

Purpose of the Study:

To investigate the precise role of Bcl-3 in adjuvant-mediated T cell survival.
To confirm adjuvant-induced expression of Bcl-3 at both mRNA and protein levels.
To elucidate the intrinsic functions of Bcl-3 in T cell activation.

Main Methods:

Confirmation of previous findings on adjuvant effects on T cell lifespan in Bcl-3-deficient models.
Quantification of Bcl-3 mRNA and protein levels following adjuvant stimulation.
Generation of transgenic mice overexpressing Bcl-3 under the human CD2 promoter.
In vitro and in vivo assessment of T cell activation kinetics in Bcl-3 overexpressing T cells.

Main Results:

Adjuvant-induced expression of Bcl-3 was confirmed at the mRNA and protein levels.
T cells overexpressing Bcl-3 exhibited delayed activation kinetics, independent of other cell types.
This delay in T cell activation occurred early in the immune response, both in vitro and in vivo.
The study confirmed that adjuvants enhance T cell survival even in the absence of Bcl-3, highlighting complexity.

Conclusions:

Bcl-3 plays a role in regulating T cell activation timing.
While Bcl-3 is upregulated by adjuvants and affects T cell lifespan, its absence does not fully abrogate adjuvant-induced T cell survival.
The intrinsic property of Bcl-3 to slow T cell activation suggests a regulatory function in immune responses beyond adjuvant pathways.