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Related Experiment Videos

A differential study between antemortem bleeding and a postmortem infiltration of hemoglobin.

K Kibayashi1, T Higashi, S Tsunenari

  • 1Department of Forensic Medicine, Kumamoto University Medical School, Japan.

Nihon Hoigaku Zasshi = the Japanese Journal of Legal Medicine
|June 1, 1991
PubMed
Summary

This study shows glycophorin detection can differentiate antemortem bleeding from postmortem hemoglobin infiltration in skin tissues for up to 12 days. This aids in forensic analysis of skin samples.

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Area of Science:

  • Forensic Pathology
  • Biochemistry
  • Immunology

Background:

  • Distinguishing antemortem bleeding from postmortem hemoglobin infiltration is crucial in forensic investigations.
  • Glycophorins, components of erythrocytic membranes, are potential biomarkers for differentiating these conditions.

Purpose of the Study:

  • To evaluate the utility of glycophorin detection in differentiating antemortem bleeding from postmortem hemoglobin infiltration in experimental skin models.
  • To determine the temporal stability of glycophorins in postmortem skin samples.

Main Methods:

  • Extraction of glycophorins from two experimental skin models: one with injected human blood (antemortem bleeding) and one with postmortem hemoglobin infiltration.
  • Utilized anti-glycophorin serum for glycophorin detection via counterimmunoelectrophoresis, double-immunodiffusion, and rocket-immunoelectrophoresis at various time points.

Related Experiment Videos

  • Compared glycophorin levels in bleeding versus hemoglobin-infiltrated samples over 18 days.

    • Main Results:

    • Glycophorins were detected in antemortem bleeding samples for up to 9 days, and faintly detected up to 12 days.
    • Quantitative analysis showed a significant decrease in glycophorin levels after 12 days post-bleeding.
    • No glycophorins were detected in postmortem hemoglobin-infiltrated or control skin samples at any time interval.

    Conclusions:

    • Glycophorin detection is a viable method for differentiating antemortem bleeding from postmortem hemoglobin infiltration in skin tissues.
    • This method is reliable for up to 9-12 days postmortem, aiding in forensic casework.
    • The absence of glycophorins in postmortem hemoglobin infiltration highlights its specificity.