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Reversible molecular switching of molecular beacon: controlling DNA hybridization kinetics and thermodynamics using

Ronghua Yang1, Jianyu Jin, Liping Long

  • 1State Key Lab of Chemo/Biosensing and Chemometrics, Biomedical Engineering Center, College of Chemistry and Chemical Engineering, Hunan University, Changsa, 410082, China. Yangrh@pku.edu.cn

Chemical Communications (Cambridge, England)
|February 12, 2009
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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...