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Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
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Dynamic cell culture: a microfluidic function generator for live cell microscopy.

Philip J Lee1, Terry A Gaige, Paul J Hung

  • 1CellASIC Corporation, San Leandro, CA, USA. pjlee@cellasic.com

Lab on a Chip
|February 12, 2009
PubMed
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This study introduces a microfluidic system for live cell microscopy, enabling precise control over solution exchange and cell culture conditions for extended periods. The system allows for dynamic delivery of various chemical exposure profiles to cells.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Live cell microscopy requires controlled environments for long-term observation.
  • Precise manipulation of chemical microenvironments is crucial for studying cellular responses.
  • Existing systems may lack dynamic control over solution exchange and cell culture conditions.

Purpose of the Study:

  • To develop a microfluidic system for time-lapsed, live cell microscopy.
  • To enable precise control over solution exchange using a dynamic flow controller.
  • To facilitate the delivery of various chemical exposure profiles to cultured cells.

Main Methods:

  • Development of application-specific microfluidic plates for adherent and non-adherent cell types.
  • Implementation of continuous medium perfusion for multi-day cell culture.

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  • Integration of upstream channels with custom software for dynamic flow control.

    • Main Results:

    • The microfluidic system successfully maintains cell viability for multiple days.
    • Dynamic flow control allows for precise delivery of diverse exposure profiles (e.g., square waves, step functions, ramps).
    • The system enables time-lapsed microscopy under precisely controlled chemical microenvironments.

    Conclusions:

    • The developed microfluidic system offers a robust platform for advanced live cell imaging.
    • Precise control over solution exchange and exposure profiles enhances cellular studies.
    • This technology supports long-term observation of cellular dynamics in response to controlled stimuli.