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Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

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[High mobility group box-1 protein induces apoptosis in mouse peritoneal macrophages]

Feng Liu1, Yong-ming Yao, Ning Dong

  • 1Department of Basic Research, Burns Institute, First Hospital Affiliated to the Chinese PLA General Hospital, Beijing 100037, China.

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
|February 13, 2009
PubMed
Abstract

Insights

High mobility group box-1 protein (HMGB1) induces apoptosis in mouse macrophages in a dose- and time-dependent manner. HMGB1 significantly increases macrophage cell death, peaking at 24 hours post-stimulation.

Area of Science:

  • Immunology
  • Cell Biology

Context:

  • High mobility group box-1 protein (HMGB1) is a nuclear protein with extracellular functions.
  • HMGB1 plays roles in inflammation and immune responses.
  • The effect of HMGB1 on macrophage apoptosis requires further elucidation.

Purpose:

  • To investigate the proapoptotic effect of HMGB1 on murine peritoneal macrophages.

Summary:

  • Murine peritoneal macrophages were exposed to varying concentrations and durations of HMGB1.
  • Apoptosis was assessed using Annexin V-PE and 7-amino-actinomycin-D staining via flow cytometry and confocal microscopy.
  • HMGB1 demonstrated a dose-dependent and time-dependent induction of apoptosis, with a peak effect at 24 hours.

Impact:

  • HMGB1 exhibits a proapoptotic effect on macrophages.
  • This finding contributes to understanding HMGB1's role in cellular regulation and immune processes.
  • Potential implications for inflammatory diseases where HMGB1 is implicated.

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