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Updated: Jun 25, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Interleukin 10 (IL-10) gene variants and susceptibility for paediatric onset Crohn's disease
1Department of Pediatrics, University of Montreal, Montreal, QC, Canada. devendra.amre@umontreal.ca
Background:
A recent genome-wide association study in adult patients with ulcerative colitis (UC) has implicated the interleukin 10 (IL-10) gene as an important candidate gene. Moreover, a UC-associated single nucleotide polymorphism (SNP) rs3024405 was also significantly associated with adult Crohn's disease (CD).
Aims:
To examine whether IL-10-CD associations extended to paediatric-onset CD.
Methods:
We implemented the case-control design at three paediatric gastroenterology clinics in Canada. CD patients (
Results:
A total of 270 patients and 336 controls were studied. The mean age (+/-s.d.) at diagnosis was 12.1 (+/-3.5). There were a slightly higher proportion of male patients (56.3%). Of the five IL-10 tag-SNPs, rs2222202 (C/T) (P = 0.03) and rs1800871 (C/T) (P = 0.05) showed significant allelic associations with CD. Specific IL-10 SNPs were associated with CD disease location and/or disease behaviour.
Conclusions:
Our gene-wide analysis replicates recent findings of associations between IL-10 and adult CD, and suggests that these associations extend to paediatric-onset CD as well.
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