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Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
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Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
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Cytoskeletal Coordination in Cell Migration

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Related Experiment Video

Updated: Jun 25, 2026

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

Soluble vascular cell adhesion molecule-1 induces human eosinophil migration.

S Ueki1, J Kihara, H Kato

  • 1Department of Clinical and Laboratory Medicine, Akita University School of Medicine, Akita, Japan.

Allergy
|February 13, 2009
PubMed
Summary

Soluble vascular cell adhesion molecule-1 (sVCAM-1) drives eosinophil migration in asthma. This study shows sVCAM-1 directly causes eosinophil movement and activation, contributing to allergic inflammation.

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Published on: March 21, 2014

Area of Science:

  • Immunology
  • Cell Biology
  • Allergy Research

Background:

  • Tissue eosinophilia is characteristic of allergic diseases like asthma.
  • Adhesion molecules are crucial for eosinophil accumulation and activation in allergic inflammation.
  • The role of soluble adhesion molecules in asthma pathophysiology requires further investigation.

Purpose of the Study:

  • To investigate the effect of soluble vascular cell adhesion molecule-1 (sVCAM-1) on eosinophil migration.
  • To elucidate the signaling pathways involved in sVCAM-1-induced eosinophil chemotaxis.

Main Methods:

  • Purification of peripheral blood eosinophils.
  • In vitro assessment of eosinophil migration in response to sVCAM-1.
  • Analysis of signaling pathways using neutralizing antibodies and signal inhibitors.
  • Bead-based multiplex assay for protein phosphorylation.

Main Results:

  • sVCAM-1 induced concentration-dependent eosinophil chemotaxis, not chemokinesis.
  • sVCAM-1 promoted eosinophil cell shape change and actin polymerization.
  • sVCAM-1-induced chemotaxis involved activation of tyrosine kinase Src, p38 MAPK, and ERK MAPK via VLA-4.

Conclusions:

  • Soluble VCAM-1 in circulation may significantly contribute to eosinophilic inflammation in asthma.
  • Understanding sVCAM-1's role provides insights into allergic airway disease mechanisms.
  • Targeting sVCAM-1 pathways could offer therapeutic strategies for eosinophilic inflammatory conditions.